Figure 7From: N-Methyl-N'-nitro-N-nitrosoguanidine-induced senescence-like growth arrest in colon cancer cells is associated with loss of adenomatous polyposis coli protein, microtubule organization, and telomeric DNAImmunohistochemical analysis of APC and α-tubulin proteins in HCT-116 cells after treatment with MNNG. Cells were grown in 0.5% FBS containing medium on cover slips and treated with different concentrations of MNNG for 50 h. Cells were fixed and processed for immunofluorescence staining as described in the Materials and Methods. Images were captured on the Zeiss upright microscope. Morphological changes were also recorded on an inverted Zeiss Microscope.Back to article page