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Figure 8 | Molecular Cancer

Figure 8

From: N-Methyl-N'-nitro-N-nitrosoguanidine-induced senescence-like growth arrest in colon cancer cells is associated with loss of adenomatous polyposis coli protein, microtubule organization, and telomeric DNA

Figure 8

Determination of telomeric DNA levels by Q-FISH analysis in HCT-116 cells treated with MNNG. HCT-116 cells were treated with 50 and 100 μM of MNNG and then processed for Q-FISH analysis as described in Materials and Methods. Panel A shows the data of a FISH analysis. Panel B shows the quantitative analysis of the FISH data of the control and MNNG-treated cells. The percentage telomeric area of the interphase nuclei in FISH preparations was quantified using a software package (Metaview Imaging system-3.6a; Universal Imaging Co., Westchester, PA). From each sample at least 50 interphase nuclei were quantified, and mean values of percent telomeric area compared to the nuclear area were calculated. The values for the treated cells were compared to the control cells.

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