Figure 3
From: Mitochondrial inhibition of uracil-DNA glycosylase is not mutagenic
Western blot analysis of mitochondrial UDG expression in transfected cell lines: Upper panel shows western blotting of mitochondrial extracts with UDG antibody the lower panel shows the same blot probed with Cox II antibody to assess for equal loading of the samples. Lane 1 is mitochondrial extract from parental MCF12A cells, lane 2 is mitochondrial extract from MCF12A cells transfected with empty pCMV/myc/mito vector, lane 3 is mitochondrial extract from MCF12A cells transfected with pCMV UNG vector, lane 4 is mitochondrial extract from MCF12A cells transfected with pCMV UGI vector. A band of lower molecular weight was seen in some extracts.