Real-Time PCR validation A. Serial dilutions of a standard cDNA duplicates for the construction of standard curves for GAPDH and Tpl2/Cot. The curve slopes shown on the upper right corner of each plot are -3.61 and -3.59 respectively. Black arrows correspond to dilution 1:10; white arrows to 1:20; left-pointed arrow is NTC (no-template control). X-axes represent the Log of the dilution factor, Y-axes the mean Ct of duplicates. B. Dissociation (melting) curve of the PCR products, showing a peak at 81.7°C for both Tpl2/Cot(black arrow) and GAPDH(white arrow), while NTCs have either no peak or a peak at a much lower temperature (thin arrow).