Determination of caspase-3 activation using Western blot analysis of caspase-3-p20 active band. Four treatment groups: CTL; 40 μM DXM for 8 h; 100 μM TMZ for 6 h; pretreatment with 40 μM DXM for 2 h followed by 100 μM TMZ for 6 h. (A) A representative gel picture showing caspase-3 activation. (B) A representative gel picture showing level of expression of β-actin. (C) Densitometric analysis showing percent change in optical density of the caspase-3-p20 active band over CTL. Significant difference between CTL and TMZ treated cells was indicated by * (P ≤ 0.05) and significant difference between TMZ treated cells and DXM plus TMZ treated cells was indicated by # (P ≤ 0.05).