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Figure 3 | Molecular Cancer

Figure 3

From: Expression of the pro-angiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 by human breast carcinomas is responsive to nutrient deprivation and endoplasmic reticulum stress

Figure 3

Response of VEGF, IL-8, GRP78, GADD153 and GAPDH mRNA expression to deprivation of various amino acids. TSE cells adapted to MEM were grown to confluence, and cultured for 12 hours in MEM without any amino acids (-13 a.a.), containing the normal supplement of amino acids (+13 a.a.), or in media in which one amino acid at a time was deleted as indicated. Total RNA was isolated and Northern blotting analysis was performed. Fold increases in mRNA levels relative to the sample containing the normal supplement of amino acids (+13 a.a.) are shown in (A).

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