Figure 4

Response of VEGF, IL-8, GRP78, GADD153, and GAPDH mRNA expression to glutamine starvation over time, and to various initial concentrations of glutamine in the media. (A) Confluent TSE cells were cultured in glutamine-free medium for the times indicated, or (B) in media containing the indicated initial concentrations of glutamine for 24 hours. Total RNA was isolated, and Northern blotting analysis was performed.