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Table 2 Comparison of inter-tube variability in transcript quantification: ACTB transcripts were amplified in multiple tubes simultaneously and the PCR reactions terminated at cycles 20, 23, 26, 29, 32 & 35. Experiments were performed in triplicate. The ACTB transcript number in all tubes was quantified separately at the designated end points and the variability between the values obtained from the multiple tubes compared. There is very little variation between the transcript numbers obtained from the multiple tubes at each end point.

From: Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

Number of cycles of amplification Mean transcript number from 3 tubes Standard deviation Coefficient of variation
20 150369 3012.87 2%
23 144224 3047.39 2.11%
26 139305 7354.84 5.28%
29 135205 996.66 0.70%
32 135138 2175.03 1.61%
35 135413 2797.04 2%