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Table 4 Comparison between StaRT PCR and Real Time PCR techniques for their discriminatory power: The ability of the 2 techniques to distinguish between different confluence patterns and starting total RNA amounts based on the expression of individual genes was examined. All experiments were performed in triplicate. The numbers represent p values & values <0.05 (values in bold) are considered significant. Both StaRT PCR and Real Time PCR techniques have comparable power to discriminate between transcriptional status of cultures cells based on transcript quantification. As hypothesized, the differences in the starting RNA amount did not influence the transcript quantification.

From: Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

  Rb E2F p16 PCNA
  StaRT PCR Real Time PCR StaRT PCR Real Time PCR StaRT PCR Real Time PCR StaRT PCR Real Time PCR
LD 419 Confluence 60% vs. 100% 0.019 0.33 0.36 0.31 0.62 0.21 <0.001 0.023
LD 419 Starting RNA amount 0.31 0.83 0.3 0.58 0.48 0.95 0.8 0.94
T24 Confluence 60% vs. 100% <0.001 0.4 0.18 0.024 0.061 0.99 0.73 0.86
T24 Starting RNA amount 0.03 0.63 0.34 0.52 0.15 0.34 0.12 0.53