Effect of PI 3-K inhibitors on IκB expression and phosphorylation in HEC-1-A, RL-95-2 and Ishikawa cells. Western analysis was performed on cell protein lysates from pooled attached and floating cells. β-actin was used as control to correct for loading. Densitometric analyses were performed using BIO RAD gel doc system and are presented as a ratio (value/β-actin). 2 × 106 cells were plated for 24 h and cultured in medium in the presence or absence of LY294402 or Wortmannin. Data represent the mean ± SEM of 4 independent experiments. * p < 0.05 compared to control.