Skip to main content

Table 7 Primer sequences for real-time RT-PCR

From: Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis

Gene Forward primer Reverse primer Accession no.
Beta-actin 5'-GACGGTCAGGTCATCACTATCG-3' 5'-ACGGATGTCAACGTCACACTTC-3' NM_031144
I-FABP 5'-AGGAAGCTTGGAGCTCATGACA-3' 5'-TCCTTCCTGTGTGATCGTCAGTT-3' K01180
Neu Oncogene 5'-GTGGTCGTTGGAATCCTAATCAA-3' 5'-CCTTCCTTAGCTCCGTCTCTTTTA-3' X03362
PYY 5'-AGGAGCTGAGCCGCTACTATGC-3' 5'-TTCTCGCTGTCGTCTGTGAAGA-3' M17523
Glucagon 5'-TGGTGAAAGGCCGAGGAAG-3' 5'-TGGTGGCAAGGTTATCGAGAA-3' K02813
Somatostatin 5'-GGAAACAGGAACTGGCCAAGT-3' 5'-TGCAGCTCCAGCCTCATCTC-3' K02248
PAP III 5'-AAGAGGCCATCAGGACACCTT-3' 5'-CACTCCCATCCACCTCTGTTG-3' L20869
CYP4F1 5'-CCAAGTGGAAACGGTTGATTTC-3' 5'-TCCTGGCAGTTGCTGTCAAAG-3' M94548
GST 5'-ACTTCCCCAATCTGCCCTACTTA-3' 5'-CGAATCCGCTCCTCCTCTGT-3' X04229
Cyclin D1 5'-TCAAGTGTGACCCGGACTGC-3' 5'-ACTTCCCCTTCCTCCTCGGT-3' D14014
Beta defensin-1 5'-TCTTGGACGCAGAACAGATCAATA-3' 5'-TCCTGCAACAGTTGGGCTATC-3' AF093536
H+, K+-ATPase 5'-ATTCCGCATCCCTAGACAACG-3' 5'-TCTTACTAAAGCTGGCCATGATGTT-3' M90398
Prostaglandin D synthetase 5'-CAAGCTGGTTCCGGGAGAAG-3' 5'-TTGGTCTCACACTGGTTTTTCCTTA-3' J04488
RBP 5'-TCGTTTCTCTGGGCTCTGGTAT- 3' 5'-TTCCCAGTTGCTCAGAAGACG-3' M10934
Muc3 5'-AAGGTGTGAGGAAGTGATGGAGA-3' 5'-GCAGAGACCGTCGGCTTTATC-3' U76551
BTEB1 5'-ACACTGGTCACCATCGCCAA-3' 5'-GGACTCGACCCAGATTCGGT-3' NM_057211
BTEB2 5'-CTACTTTCCCCCATCACCACC-3' 5'-GAATCGCCAGTTTCGAAGCA-3' AB096709