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Table 3 PCR primers for amplification; all primers are designed for the sense strand.

From: A cluster of genes located in 1p36 are down-regulated in neuroblastomas with poor prognosis, but not due to CpG island methylation

Gene Primer Sequence Length of fragment (bp) Accession number
UBE4B FP 5'-TTGTTAGTTTATTTGGTTTAGGTT-3' 466 NM_006048
  RP 5'-TAACAAAACCCAACACTATAAAAAAAACCCCT-3'   
KIF FP 5'-TTTTTAAGGGTATTTTTTAGAAGGG-3' 644 NM_015074
  RP 5'-ACTATAACCAATCACAACACAAAACTC-3'   
PGD FP-A 5'-GTGAGTTGTTATGGTTATAGTTG-3' 301 NM_002631
  FP-B 5'-ATGGTGTGGTTTTATGGTTTTATTT-3'   
  RP 5'-CAAAATCACAAAACCCCAAATAA-3'   
APITD1 1FP-A 5'-GATTTTGTAAGATATATTTGAGGTAT-3' 231 chr1_29_927.b
  1FP-B 5'-ATGGAGTTTTTGATAATGTGTATTG-3'   
  1RP 5'-AACCCCCTACTCAACTTACTCTAC-3'   
  2FP-A 5'-ATTAGGTTTTGGGGTGTAGTAGTGAT-3' 199  
  2FP-B 5'-GTAGAGTAAGTTGAGTAGGGGGTTG-3'   
  2RP 5'-ACCCTAAACAAAAACAAAAAAAC-3'   
  3FP-A 5'-GTAGAGTAAGTTGAGTAGGGGGTTG-3' 350  
  3FP-B 5'-TTGTTTTTGTTTAGGGTCGGTT-3'   
  3RP 5'-CAAAACCAAAAAATAACCTCTC-3'   
DFFA FP 5'-AAGTTAAAAATAATTTTTAGGTTGAAT-3' 407 NM_004401
  RP 5'-ACCAACCCTTACTCCTCAAATCT-3'   
PEX14 FP 5'-TGATTAGTTAGGTTTTAGAAAGATGG-3' 333 NM_004565
  RP 5'-CAAATAAAACCAAAAATACTAACAAAC-3'   
  1. Column 2: FP, forward primer; RP, reverse primer; FP-A and FP-B, primers used for semi-nested PCR together with the same reverse primer. Column 5: UCSC Genome Browser August 2001 draft sequence was used as reference sequence (URL: http://genome.ucsc.edu).