Figure 4From: Inactivation of MAP kinase signalling in Myc Transformed Cells and Rescue by LiCl inhibition of GSK3Myc protein turnover after the addition of LiCl in Myc transformed and non transformed fibroblasts. (A) Endogenous Myc protein in Li+ and K+ treated non-transformed fibroblasts. This was identical to fibroblasts transfected with empty vector. Panel 1 is the scanned image of a western blot autoradiograph. Panel 2 shows the half life values of the Myc protein under the different conditions. CEF represents the non-transformed control fibroblasts (B) Turnover of the Myc protein in Li+ and K+ treated c-Myc transformed fibroblasts. Panel 1 is the scanned image of a western blot autoradiograph. Panel 2 shows the half life values of the Myc protein under the different conditions. CEF represents the non-transformed control fibroblasts (C) Turnover of the Myc protein in Li+ and K+ treated v-Myc transformed fibroblasts. Panel 1 is the scanned image of a western blot autoradiograph. Panel 2 shows the half life values of the Myc protein under the different conditions. CEF represents the non-transformed control fibroblasts. The experiments were independently repeated three times and one representative experiment is shown.Back to article page