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Figure 3 | Molecular Cancer

Figure 3

From: Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer

Figure 3

Expression of Hsulf-1 in pancreatic cancer cell lines (A) Quantification of Hsulf-1 mRNA levels in pancreatic cancer cell lines by real time QRT-PCR as described in the Materials and Methods section. Values are normalized to housekeeping genes (cyclopilin B and HRPT), and presented as mean ± SD. (B/C) Panc-1 cells were stable transfected with a Hsulf-1 sense expression plasmid as described in the Material and Methods section. (B) Hsulf-1 sense RNA expression in Panc-1 cells was verified by Northern blot analysis using a radiolabeled Hsulf-1 antisense riboprobe. A sample Northern blot of 2 controls and 2 transfected clones is shown. (C) Expression of c-myc tagged Hsulf-1 (arrow) by immunoblot analysis as described in the Materials and Methods section. Equal loading of the protein samples was confirmed using anti-γ-tubulin antibodies. (D) Sulfatase activity was measured as described in Material and Methods section in control and positive transfected clones. Data are expressed as relative fluorescence and presented as mean ± SD.

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Molecular Cancer

ISSN: 1476-4598