Table 2 RT-PCR primers and conditions
From: Amplification and overexpression of the ID4 gene at 6p22.3 in bladder cancer
Designation | Sequence | Tm(°C) | Product size (bp) |
|---|---|---|---|
GAPDH 350s | TCCCATCACCATCTTCCA | 62.3 | 379 |
GAPDH 350as | CATCACGCCACAGTTTCC | 61.9 | Â |
Aktin661S | TGACGGGGTCAC | 72.2 | 661 |
Aktin661AS | CTAGAAGCATTT | 70.9 | Â |
ID4 fw | CCGCCCAACAAGAAAGTCAG | 59.4 | 188 |
ID4 rv | GGTGTTGAGCGCAGTGAG | 58.2 | Â |
E2F3 fw | ACGTCTCTTGGTCTGCTCAC | 59.4 | 155 |
E2F3 rv | TCTTAATGAGGTGGATGCCT | 55.3 | Â |
DEK fw | GTGGGTCAGTTCAGTGGC | 58.2 | 291 |
DEK rv | AGGACATTTGGTTCGCTTAG | 55.3 | Â |