S3c expression inhibited RAR-β and -γ expression and increased expression of RAR-α in NRP-152 cells. Panel A: Effect of S3c on RAR-β mRNA levels. Panel B: Effect of S3c on RAR-γ mRNA levels. Panel C: Effect of S3c on RAR-α mRNA levels. NRP-152, NRP-154, NRP-pBABE, and 152-S3c cells were grown to confluence, and RNA was harvested as described in Materials & Methods. Electrophoretic separation of RNA was followed by transfer to nitrocellulose, then hybridization with 32P-labeled probe, followed by autoradiography. Lane 1 = NRP-152 (rat benign prostatic hyperplasia line); lane 2 = NRP-154 (rat prostatic carcinoma line); lane 3 = 152-pBABE; lane 4 = 152-S3c. The comparison to 18S RNA is shown for each.