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Table 2 Androgen-Insensitivity is conferred by S3C expression in BPH-1 cells

From: Stable expression of constitutively-activated STAT3 in benign prostatic epithelial cells changes their phenotype to that resembling malignant cells

Cell nM DHT %Stimulation μM F + nM DHT %Inhibition
BPH 10 200 1 10 97
BPH-pIRES 10 250 1 10 99
BPH-S3c 10 2 1 10 -4
DU145 10 3 1 10 3
  1. Cells were grown in 96-well plates for 72 hr, in the presence or absence of drugs (DHT = dihydrotestosterone; F = flutamide, bolded and underlined) as indicated. The MTT assay was used to assess proliferation. %Enhancement = (absorbance + drug/absorbance - drug) × 100; % Inhibition = 1-(absorbance + drug/absorbance - drug) × 100. The responses of DU145 cells, a human prostate cancer cell line that is androgen-insensitive and resistant to flutamide, is shown for comparison.