Stable expression of constitutively-activated STAT3 in benign prostatic epithelial cells changes their phenotype to that resembling malignant cells

Table 2 Androgen-Insensitivity is conferred by S3C expression in BPH-1 cells

Cell	nM DHT	%Stimulation	μM F + nM DHT		%Inhibition
BPH	10	200	1	10	97
BPH-pIRES	10	250	1	10	99
BPH-S3c	10	2	1	10	-4
DU145	10	3	1	10	3

Cells were grown in 96-well plates for 72 hr, in the presence or absence of drugs (DHT = dihydrotestosterone; F = flutamide, bolded and underlined) as indicated. The MTT assay was used to assess proliferation. %Enhancement = (absorbance + drug/absorbance - drug) × 100; % Inhibition = 1-(absorbance + drug/absorbance - drug) × 100. The responses of DU145 cells, a human prostate cancer cell line that is androgen-insensitive and resistant to flutamide, is shown for comparison.

ISSN: 1476-4598