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Table 3 NRP-152 Cells Transfected with S3c lost sensitivity to JAK2 inhibitor AG490

From: Stable expression of constitutively-activated STAT3 in benign prostatic epithelial cells changes their phenotype to that resembling malignant cells

Cell

S3c?

Rx

μM

% Apoptotic +/- SD

NRP-154

YES

AG490

0

8 ± 4

   

100

7.5 ± 5

5152-S3c

YES

AG490

0

7.5 ± 4

   

100

6.3 ± 3

152-pIRES

NO

AG490

0

11 ± 2

   

100

38 ± 5*

NRP-152

NO

AG490

0

7.5 ± 4

   

100

45 ± 10*

  1. Cells were placed in 60 mm wells for 48 hr with compound at the concentrations indicated. Zero concentration of the compound is the vehicle (DMSO) control. At the end of the incubation period, cells were harvested, washed, and stained with FITC-annexin V, to demonstrate apoptotic cells. Quantification of fluorescence was performed on a Becton-Dickinson FACScan flow cytometer using CellQuest software.
  2. * p < 0.005 by Student t-test, compared to vehicle-treated cells.