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Table 3 NRP-152 Cells Transfected with S3c lost sensitivity to JAK2 inhibitor AG490

From: Stable expression of constitutively-activated STAT3 in benign prostatic epithelial cells changes their phenotype to that resembling malignant cells

Cell S3c? Rx μM % Apoptotic +/- SD
NRP-154 YES AG490 0 8 ± 4
    100 7.5 ± 5
5152-S3c YES AG490 0 7.5 ± 4
    100 6.3 ± 3
152-pIRES NO AG490 0 11 ± 2
    100 38 ± 5*
NRP-152 NO AG490 0 7.5 ± 4
    100 45 ± 10*
  1. Cells were placed in 60 mm wells for 48 hr with compound at the concentrations indicated. Zero concentration of the compound is the vehicle (DMSO) control. At the end of the incubation period, cells were harvested, washed, and stained with FITC-annexin V, to demonstrate apoptotic cells. Quantification of fluorescence was performed on a Becton-Dickinson FACScan flow cytometer using CellQuest software.
  2. * p < 0.005 by Student t-test, compared to vehicle-treated cells.