Figure 2
From: Regulation of pancreatic cancer cell migration and invasion by RhoC GTPase and Caveolin-1
RhoC GTPase expression in BxPC-3 and HPAF-II cell lines. A. Total GDP/GTP-bound RhoC was measured in a panel of 10 PC cell lines. Protein (30 μg) was harvested from actively growing PC cell lines. B. Expression of RhoC in BXPC-3 and HPAF-II cells was measured by RT-PCR and immunoblot analysis using RhoC-specific primers and antibody, respectively. To determine the amount of active, GTP-bound RhoC a Rho activation assay was performed. GST-rhotekin is used to pull down RhoC-GTP from total cell lysates followed by immunoblotting with a RhoC-specific antibody. C. The RhoC activation assay was performed on the GFP-cav-1 HPAF-II transfectants 24 h after transfection. Differences in active and total RhoC levels were measured by densitometry and are represented.