Figure 3
From: Regulation of pancreatic cancer cell migration and invasion by RhoC GTPase and Caveolin-1
Establishment of stable, dominant negative RhoC HPAF-II transfectants. A. Results of a RhoC activation assay and total RhoC Western blot comparing wildtype, C3 exotransferase treated, vector control and dominant negative RhoC (dnRhoC) transfected HPAF-II cells. B. Comparison of total (dark grey) and active (light grey) RhoA levels utilizing a RhoA-specific antibody. C. Results of the effect of dnRhoC transfection and C3 treatment on HPAF-II cells in a colloidal gold migration assay. Inhibition of RhoC lead to a significant decrease in migration (*p = 0.001) and invasion (*p = 0.023).