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Figure 4 | Molecular Cancer

Figure 4

From: Regulation of pancreatic cancer cell migration and invasion by RhoC GTPase and Caveolin-1

Figure 4

Interaction of cav-1 and RhoC in BxPC-3 and HPAF-II PC cell lines. A. Aliquots of 500 μg total protein from BxPC-3, HPAF-II and HPAF-II/dnRhoC transfectants were immunoprecipitated with either a monoclonal or polyclonal antibody to cav-1. Proteins were separated by SDS-PAGE, transferred to nitrocellulose and probed with either a RhoC-specific or cav-1 antibody. Normal IgG negative controls are also shown. B. Immunoblot analysis of 50 μg of total protein for total cav-1 expression using a cav-1 specific monoclonal antibody and actin was measured as a loading control. Densitometry was performed on each blot and expression levels were normalized to the actin control. Expression levels are represented as arbitrary units (AU).

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