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Figure 4 | Molecular Cancer

Figure 4

From: Telomerase inhibition by siRNA causes senescence and apoptosis in Barrett's adenocarcinoma cells: mechanism and therapeutic potential

Figure 4

Senescence following treatment of SEG-1 cells with telomerase specific siRNAs. SEG-1 cells were transfected with either control or telomerase specific siRNAs and cultured for three weeks. For senescence assay, cells were rinsed in PBS and stained overnight in solution containing X-gal. Expression of β-galactosidase was monitored using an Olympus® fluorescence microscope. Panels: A I. SEG-1 cells transfected with control siRNA, evaluated for of β-galactosidase expression. A II. SEG-1 cells transfected with telomerase siRNA, evaluated for of β-galactosidase expression. Expression of β-galactosidase is seen as blue colour. A III. Typical senescence associated morphology in a subset of SEG-1 cells transfected with telomerase siRNAs. B I. Normal cells from stomach and intestine, transfected with control siRNA and evaluated for of β-galactosidase expression. B II. Normal cells from stomach and intestine, transfected with telomerase specific siRNAs and evaluated for of β-galactosidase expression.

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