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Figure 6 | Molecular Cancer

Figure 6

From: Telomerase inhibition by siRNA causes senescence and apoptosis in Barrett's adenocarcinoma cells: mechanism and therapeutic potential

Figure 6

Induction of apoptosis proteins following treatment of SEG-1 cells to telomerase specific siRNAs. SEG-1 cells, transfected with control (Cont) or telomerase specific (Tel) siRNAs were cultured for two weeks and analyzed for protein expression by western blotting. The expression of p63, TNF-related apoptosis inducing ligand (TRAIL), TNF ligand superfamily, member 6 (FASL), and p16 was monitored using mouse monoclonal anti-p63 (Zymed), rabbit polyclonal anti-TRAIL (Santa Cruz), mouse monoclonal anti-FASL (Cell Science), and mouse monoclonal anti-p16 (Sigma) antibodies, respectively. A. Western blot showing expression of p63 and TRAIL in control (Cont) and telomerase (Tel) siRNA treated cells. B. Bar graph showing relative expression of p63 and TRAIL following normalization with glyceraldehyde-3-phosphate dehydrogenase (GAPDH). C. Western blot showing expression of FASL and p16 in control (Cont) and telomerase (Tel) siRNA treated cells. D. Bar graph showing relative expression of FASL and p16 following normalization with glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

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