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Figure 1 | Molecular Cancer

Figure 1

From: Overcoming cisplatin resistance by mTOR inhibitor in lung cancer

Figure 1

Apoptosis assay using annexin-V by FACS analysis in cisplatin resistant cell line (SR-2). Cells were treated with cisplatin alone or in combination with 0.15 ug of CCI-779. Left panels are dual staining for propidium iodide uptake and annexin-VFITC, Right panels are corresponding distribution of annexin VFITC staining in populations of cells. 1A: control. 1B: SR-2 treated with 6 ug of cisplatin (No annexin VFITC was detected) 1C: SR-2 treated with 6 ug of cisplatin+CCI-779 (small peak of annexin VFITC was detected) 1D: SR-2 treated with 10 ug of cisplatin (a peak of annexin VFITC was detected) 1E: SR-2 treated with 10 ug of cisplatin+CCI-.779 (a large peak of annexin VFITC was detected) Note: CCI-779 alone at 0.15 and 0.3 ug/ml did not induce apoptosis (data not shown).

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