Figure 3

Induction of COX-2 and α-smooth muscle actin (SMA) protein in pancreatic stellate cells (PSCs). After isolation of PSCs, equal amounts of protein from the cell lysates were loaded by SDS-PAGE and immunoblotted with COX-2 or α-SMA antibodies. Upper panels show representative western blots and lower panels show the densitometry data from all experiments. PSCs expressed α-SMA after seven days in culture. In contrast, PSCs expressed COX-2 throughout this time period.