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Table 3 Comparison of gene expression in A549 and 12.2 cell lines. Genes expressed differentially between A549 and 12.2 cells were identified by qPCR, subtractive hybridization (SH), and/or TransSignal DNA-protein array (TS) as indicated. Relative quantification differences were determined for those genes analyzed by either qPCR or TS. ND, not determined.

From: Tumor suppressor in lung cancer 1 (TSLC1) alters tumorigenic growth properties and gene expression

Gene

Up-Regulated

Assay

Fold Expression Difference

Functional Class

Fibrinogen beta chain (FGB)

A549

SH/qPCR

2737.5+/-1058.5

Adhesion

Fibrinogen gamma chain (FGG)

A549

SH

ND

Adhesion

Adenomatosis polyposis coli (APC)*

A549

qPCR

1.7+/-0.2

Cellular Growth

Cyclin D1 (CCND1)†

A549

qPCR

1.6+/-0.0

Cellular Growth

β-catenin 1 (CTNNB1)*

12.2

qPCR

1.1+/-0.0

Cellular Growth

Dishevelled 1 (DVL1)*

A549

qPCR

1.1+/-0.3

Cellular Growth

v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS)†

A549

qPCR

1.4+/-0.3

Cellular Growth

Lymphoid enhancer-binding factor 1 (LEF1)*

12.2

qPCR

3.4+/-0.4

Cellular Growth

v-myc myelocytomatosis viral oncogene homolog (MYC)†

A549

qPCR

1.3+/-0.2

Cellular Growth

Interleukin 23-alpha (p19)†

A549

qPCR

1.0+/-0.0

Cellular Growth

Tumor protein p53 (TP53)†

A549

qPCR

1.7+/-0.3

Cellular Growth

Retinoblastoma 1 (RB1)†

12.2

qPCR

1.3+/-0.4

Cellular Growth

S100 calcium-binding protein P (S100P)

A549

SH/qPCR

1680.0+/-955.4

Cellular Growth

Transcription factor 4 (TCF4)*

12.2

qPCR

6.8+/-2.0

Cellular Growth

Transcription factor 7-like 2 (TCF7L2)*

12.2

qPCR

2.2+/-0.7

Cellular Growth

Transmembrane 4 superfamily member (TM4SF4)

A549

SH/qPCR

475.2+/-328.0

Cellular Growth

Centromere protein E (CENPE)

A549

SH

 

Cellular Growth

Heat shock protein 70B (HSPA6)

A549

SH/qPCR

2.4+/-0.8

Chaperone

Insulin-like growth factor binding protein 1 (IGFBP1)

A549

SH/qPCR

15.9+/-7.4

Decidualization [41]

Retinoid X receptor RXR (DR-1)

A549

TS

2.7

Decidualization [42]

Aldehyde dehydrogenase 1 (ALDH1)

A549

SH/qPCR

4.2+/-0.1

Decidualization-Implantation [43]

Annexin A2 (Lipocortin II) (ANXA2)

12.2

SH/qPCR

3.1+/-1.0

Decidualization-Implantation [43]

Metallothionein-IF (MTIF)

12.2

SH

ND

Decidualization-Implantation [43]

Metallothionein-IG (MT1G)

12.2

SH/qPCR

37.1+/-10.4

Decidualization-Implantation [43]

Cadherin 11 (OB-cadherin, osteoblast) (CDH11)

A549

SH/qPCR

5.6+/-2.7

Decidualization-Luteal/Adhesion [34,44]

Fibroblast growth factor 9 (FGF9)

A549

SH

ND

Decidualization-Proliferation [45]

Promyelocytic leukemia gene (PML)

A549

SH

ND

Decidualization-Proliferation

Similar to aldehyde dehydrogenase 6 (ALDH1A3)

A549

SH

ND

Dehydrogenase

3-alpha hydroxysteroid dehydrogenase type II (AKR1C3)

A549

SH

ND

Dehydrogenase

Dihydrodiol dehydrogenase 2 (AKR1C2)

A549

SH/qPCR

22.9+/-2.4

Dehydrogenase

Kinesin 2, light chain (KNS2)

12.2

SH/qPCR

3.9+/-0.0

Intracellular Trafficking

Matrix metalloproteinase 1 (MMP1)

12.2

qPCR

1.2+/-0.4

Invasion

Vascular endothelial growth factor (VEGF)

12.2

qPCR

4.2+/-0.3

Invasion

Ferritin, heavy chain (FTH1)

12.2

SH/qPCR

2.7+/-1.7

Iron Binding

Metallothionein-IE (MT1E)

12.2

SH/qPCR

14.8+/-8.5

Metal Homeostasis

Metallothionein-IH (MT1H)

12.2

SH

ND

Metal Homeostasis

Metallothionein-IL (MT1L)

12.2

SH/qPCR

5.8+/-0.9

Metal Homeostasis

Metallothionein-IR (MT1R)

12.2

SH

ND

Metal Homeostasis

Manganese-containing superoxide dismutase (SOD2)

A549

SH/qPCR

2.0+/-0.6

Mitochondrial

Microsomal glutathione transferase (MGST1)

A549

SH/qPCR

2.3+/-0.1

Mitochondrial

NAD(P)H menadione oxidoreductase 1, dioxin-inducible (NQO1)

A549

SH/qPCR

3.1+/-0.1

Mitochondrial

Solute carrier family 25 member 5 (SLC25A5)

A549

SH/qPCR

3.4+/-0.3

Mitochondrial

Myelin proteolipid protein (PLP)

A549

SH

ND

Myelin Constituent

Ribosomal protein S6 (RPS6)

12.2

SH/qPCR

2.5+/-1.2

Protein Kinase

v-ets erythroblastosis virus E26 oncogene homolog 2 (ETS2)

A549

qPCR

1.2+/-0.0

Ras-Induced Senescence [29]

Metallothionein-II (MTII)

12.2

SH

ND

Ras-Induced Senescence [29]

Ras induced senescence 1 (RIS1)

12.2

SH/qPCR

80.2+/-62.3

Ras-Induced Senescence [29]

Tissue inhibitor of metalloproteinase 1 (TIMP1)

12.2

qPCR

2.2+/-0.1

Ras-Induced Senescence [29]

CAAT box general (CBF)

12.2

TS

2.5

Transcription Factor

CCAAT displacement protein (CDP)

12.2

TS

2

Transcription Factor

E2F transcription factor 1 (E2F-1)

12.2

TS

2.4

Transcription Factor

Early growth response (EGR)

12.2

TS

3.4

Transcription Factor

Estrogen receptor (ERE)

12.2

TS

2.5

Transcription Factor

GATA binding protein (GATA)

12.2

TS

2.1

Transcription Factor

Glucocorticoid receptor (GRE)

12.2

TS

3

Transcription Factor

Nuclear factor of activated T-cells, cytoplasmic (NF-ATc)

12.2

TS

3.1

Transcription Factor

Signal transducer and activator or transcription 3 (STAT3)

A549

TS

2

Transcription Factor

Signal transducer and activator or transcription 4 (STAT4)

A549

TS

2.3

Transcription Factor

Upstream transcription factor (USF-1)

A549

TS

2.5

Transcription Factor

Transcription co-activator Sp110

A549

SH

ND

Transcription Factor

Similar to eukaryotic translation initiation factor 2B, subunit 1 (EIF2B1)

A549

SH

ND

Translation

Insulin-like 4 (INSL4)

A549

SH/qPCR

27.0+/-15.8

Trophoblast [46]

Keratin 8 (KRT8)

A549

SH

ND

Trophoblast [47]

cDNA clone DKFZp761C1(AL157447)

A549

SH

ND

Unknown

cDNA clone FLJ20643 (AK000650)

A549

SH

ND

Unknown

FLJ14639 (NM_032815)

A549

SH

ND

Unknown

Hit clone 451B21 (AL033522)

A549

SH

ND

Unknown

HSA1p34 genomic sequence (AL009181)

A549

SH

ND

Unknown

Rhomboid family 1 (Z69719) (RHBDF1)

A549

SH

ND

Unknown

RP11-2H8 (AC089984)

A549

SH

ND

Unknown

RP11-389E6 (AL359836)

A549

SH

ND

Unknown

RP11-478J18 (AC011700)

A549

SH

ND

Unknown

RP11-7F24 (AC018841)

A549

SH

ND

Unknown

RP1-20C7 (AL136304)

A549

SH

ND

Unknown

SR+89 (Z69706)

A549

SH

ND

Unknown

HSA14 genomic sequence (AL135745)

12.2

SH

ND

Unknown

  1. * Wnt/β-catenin pathway
  2. †G1/S transition