Analysis of bFGF, VEGF and IL-8 expression in tumors and other tissues. Tumors and other tissues were excised from the animals injected with HEK293 cells stably transfected with PTTG1 (clone 1 and clone3) and homogenized. bFGF, VEGF and IL-8 in the homogenates were analyzed by ELISA. Each analysis was performed in triplicate tissue and was normalized to mg of protein. Error bars represent ± SEM of three independent experiments.