Analysis of expression of PTTG bFGF, VEGF and IL-8 mRNAs from tumors and other tissues collected from mice subcutaneously injected with HEK293 cells stably expressing PTTG1. A: RT-PCR analysis and, B: Western blot analysis. T1: tumor 1, T2: tumor 2, M: HEKmPTTG1-2, H: heart, K: Kidney, Li: liver, Lu: lung. GAPDH and β-actin were used as control to examine equal loading. The gels are representative of two independent experiments.