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Table 2 Primer sequences and PCR conditions for the amplification of PTTG1, bFGF, VEGF, IL-8, m-PTTG1 and GAPDH.

From: Ectopic expression of PTTG1/securin promotes tumorigenesis in human embryonic kidney cells

 

Sense Primer

Antisense Primer

PCR Conditions

PTTG

ATGGCTACTCTGATCTAT

AAAATCTATGTCACAGCAAAC

95°C 5 min, 95°C 30 s, 54°C 30 s, 72°C 30 s. 28 cycles.

bFGF

TTCTTCCTGCGCATCCACCC

CTCTTAGCAGACATTGGAAG

95°C 5 min, 95°C 30 s, 56°C 30 s, 72°C 30 s. 26 cycles.

VEGF

GAATCATCACGAAGTGGTGA

AACGCGAGTCTGTGTTTTTG

95°C 5 min, 95°C 30 s, 56°C 30 s, 72°C 30 s. 28 cycles.

IL-8

ACCACCGGAAGGAACCATCT

GAATTCTCAGCCCTCTTCAA

95°C 5 min, 95°C 30 s, 58°C 30 s, 72°C 30 s. 28 cycles.

GAPDH

TGATGACATCAAGAAGGTGGT

TCCTTGGAGGCCATGTGGGCC

95°C 5 min, 95°C 30 s, 54°C 30 s, 72°C 30 s. 26 cycles.

mPTTG

GATGCTCTCCGCACTCTGGGAATCCAATCTG

TTCACAAGTTGAGGGGCGCCCAGCTGAAACAG

95°C 5 min, 95°C 30 s, 54°C 30 s, 72°C 30 s. 35 cycles.