Figure 5
From: Global gene expression profiling of cells overexpressing SMC3
Transcriptional activity in control and SMC3 overexpressing cells. Cells were seeded in 12 well plates and used at 70% confluence. Treatment groups, each as triplicate samples, were transfected with 0.1 μg/ml of the indicated reporter vector together with 0.01 μg/ml of PH-RL transfection control vector using 5 μl/ml Lipofectamine. The reporter vectors harbor multiple repeats of the consensus sequence for different transcriptional binding sites which drive the expression of a firefly luciferase. Transactivation activity was calculated based on the firefly luciferase level correcting for the transfection efficiency using the renilla luciferase level in a dual luciferase assay. Data shown are the mean ± SD from three independent determinations. * p < 0.01 SMC3-overexpressing vs. control.