FAK and β1 integrin subunit interaction in AsPC-1, BxPC-3, and Capan-2 cells. After incubating on Coll IV with 10 ng/ml IL-1α or 0.5 μg/ml anti-β1 integrin antibodies for 24 h, cells were added (2 × 105cells/well) to each well and incubated at 37°C and 5% CO2 for 30 min. After removing unattached cells, total cell lysates were immunoprecipitated with antibodies against β1 integrin subunit. Samples were resolved in 10 % SDS-PAGE gel under nonreducing conditions and transferred to polyvinylidene difluoride membranes. Membranes were then probed with anti-FAK or anti-β1 integrin antibodies. Samples from cells incubated on 3 % BSA were served as control.