Phosphorylation of FAK in AsPC-1, BxPC-3, and Capan-2 cells. After incubating on Coll IV with 10 ng/ml IL-1α and/or 60 μM Genistein for 24 h, cells were added (2 × 105 cells/well) to each well and incubated at 37°C and 5 % CO2 for 15, 30, or 60 min. After removing unattached cells, cells were collected from each time point and lysed by lysis buffer and immunoprecipated with FAK antibody as described in Methods and materials. Effect of IL-1α and Genistein on Coll IV mediated phosphorylation of FAK was demonstrated. IP, immunoprecipitation; WB, Western blot.