Figure 4

Involvement of FAK phosphorylation and integrin signaling with adhesion and invasion of pancreatic cancer cells. (A) AsPC-1, BxPC-3, and Capan-2 cells were incubated with 10 ng/ml IL-1α, with 10 ng/ml IL-1α and 60 μM Genistein, with 10 ng/ml rIL-1α and 25 μM PD98059, or with 10 ng/ml rIL-1α and equivalent amounts of DMSO vehicle for 24 h. FAK siRNA transfected cells were cultured with 10 ng/ml IL-1α for 24 h. After incubating for 30 min with/without anti-β₁ antibody, cell adhesion assay was performed at 37°C for 30 min. Statistical significance was tested by one-way analysis of variance and post hoc test (Turkey Kramer multiple comparisons). The p- values indicate statistical significance between data in controls and each treatment. Bars indicate the s.d.*: p < 0.05. (B) After incubation for 30 min with/without antibody against β₁ integrin, AsPC-1, BxPC-3, and Capan-2 cells were cultured with 10 ng/ml IL-1α, with 10 ng/ml IL-1α and 60 μM Genistein, with 10 ng/ml rIL-1α and 25 μM PD98059, or with 10 ng/ml rIL-1α and equivalent amounts of DMSO vehicle for 24 h in the inner chamber coated with Coll IV. FAK siRNA transfected cells were cultured with 10 ng/ml IL-1α for 24 h in the inner chamber. Statistical significance was tested by one-way analysis of variance and post hoc test (Turkey Kramer multiple comparisons). The p-values indicate statistical significance between data in controls and each treatment. Bars indicate the s.d. *: p < 0.05.