DNA copy number by FISH. (A) FISH analysis with 59 BACs on interphase nuclei from liposarcoma LS21. The BACs are listed from the centromeric to the telomeric side. The colour of the shading indicates the range of signals observed per nucleus, and the area the percentage of nuclei within each group of signals. The localisation of APOA2 in BAC RP11-297K8 is indicated, as well as the distance position of some of the BACs (the intervals are not equal since the BACs are not distributed evenly throughout the region). The two BACs used further in Figure 1B are highlighted, and the BACs used for array CGH in Figure 2 are marked with an asterisk (*). There is a gap of approximately 200 kb between BACs RP11-297K8 and RP11-5K23. After these analyses were performed, the gap was covered by the BAC RP11-122G18. Since no more material is available from LS21, this BAC has not been tested. (B) FISH analysis with BACs RP11-110D4 and RP11-195G14 on interphase nuclei from 10 sarcomas (LMS, leiomyosarcoma; LS, liposarcoma; MFH, malignant fibrous histiocytoma; MS, malignant schwannoma, suffix 'x', xenograft). (C) FISH analysis with four BACs proximal and three distal of RP11-110D4 and RP11-195G14 on interphase nuclei from LS3x, LS21 and MS8x.