Gene expression level by quantitative real-time RT-PCR. Relative expression level of the genes ATF6, DUSP12, GRP78 and GRP94 in 10 sarcomas. The expression levels have been normalized with the average expression of the endogenous control genes B2M, GAPDH and TBP. The average relative expression level of the tumours with normal copy number of ATF6 and DUSP12 (LS2, LS6 and MFH36) has been set to 1. The relative copy number of ATF6 and DUSP12 determined by Southern blot analysis (Figure 3) is also shown.