Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: p21WAF1 expression induced by MEK/ERK pathway activation or inhibition correlates with growth arrest, myogenic differentiation and onco-phenotype reversal in rhabdomyosarcoma cells

Figure 4

MEK/ERK pathway sustains p21 WAF1 expression. (A) RD cells were left untreated (-) or treated with TPA in the absence or in the presence of U0126 for the times indicated. (B) RD cells were transfected with the constitutively active form of HA-tagged-MEK1 (ca MEK1), -MEK2 (ca MEK2) or with the empty vector (CMV). (C) RD cells were transfected with control siRNA (siRNA-C) or ERK1 and ERK2 siRNAs (siRNAERK1-2) and then left untreated (-) or treated with TPA (+) for 4 days. Immunoblots of total lysates were performed using specific antibodies capable of recognising p21WAF1, total and phosphorylated ERK1/2, hemagglutinin (HA) and α-tubulin. The data shown are representative of three independent experiments for A and two for B and C.

Back to article page

Molecular Cancer

ISSN: 1476-4598