Figure 4

LIP overexpression is incompatible with cell growth. MCF10A cells were infected with LZRS-GFP virus (□), LZRS-HisC/EBPbeta-2-IRES-GFP virus (Δ) or LZRS-HisLIP-IRES-GFP virus (○) and monitored for maintainence of GFP expression for 3 weeks post infection. Results are shown as the mean percentage of GFP cells observed in 4–7 randomly selected microscopic fields per time point ± SEM. The loss of GFP expression observed in the LIP/GFP infected culture was determined to be very significant with a p value < 0.0001. Statistical analysis of the linear trend for the GFP only and C/EBPbeta-2/GFP infected cell populations was not significant (p values > 0.05).