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Figure 5 | Molecular Cancer

Figure 5

From: C/EBPbeta-2 confers EGF-independent growth and disrupts the normal acinar architecture of human mammary epithelial cells

Figure 5

Exogenous LIP is lost in growing MCF10A cultures. Cell extracts were prepared from uninfected MCF10A cells (lane 1), GFP positive cells sorted by flow cytometry (lanes 2, 3, & 4) infected with either LZRS-GFP virus (lane 2) or LZRS-HisLIP-IRES-GFP virus (lanes 3 & 4). The sorted cells were placed in culture and allowed to expand. Extracts were analyzed from cell samples collected at 7 days (lane 4) or 21 days (lanes 2,3) post sort. Cell extracts were also prepared from a floating subculture of MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP (lane 5) 12 days after the C/EBPbeta-2/GFP overexpressing subculture was established. Western analyses of the extracts is shown probed with anti-T7 epitope tag antibody (A) or anti-C/EBPbeta C-terminal antibody (B).

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Molecular Cancer

ISSN: 1476-4598