Figure 6

Morphology of MCF10A acini cultured on Matrigel basement membrane. Untreated MCF10A cells (A–D), MCF10A cells infected with LZRS-GFP virus (E–H), unselected MCF10A cells infected with LZRS-His-C/EBPbeta-2-IRES-GFP virus (I–L), and a floating subculture of MCF10A cells derived from the aforementioned LZRS-His-C/EBPbeta-2-IRES-GFP culture (M–P) were overlayed on Matrigel and cultured for 9 days. Light micrographs (A, C, D, E, G, I, K, M, & O) and the corresponding flurorescent images are provided to the left (B, F, H, J, L, N, & P). Since untreated MCF10A cells do not express GFP, only one fluorescent image (B) is provided to demonstrate background fluorescence. All cells were cultured at the same passage number and imaged at the same magnification. Bar represents 100 microns.