Figure 7

C/EBPbeta-2 overexpression disrupts three-dimensional acinar structure of MCF10A cells. MCF10A cells were infected with LZRS-GFP (A & B) or LZRS-His-C/EBPbeta-2-IRES-GFP virus (C & D), overlayed atop a layer of matrigel, and allowed to grow. After 19 days in culture, cells were fixed and stained with ethidium homodimer dye (A & C) to visualize nuclei (shown in red) or Alexa 594 cojugated phalloidin dye (B & D) to visualize f-actin distribution (shown in red). The GFP expression (shown in green) indicates infected cells (A–D). Images were analyzed by confocal microscopy and represent the equitorial cross sections of the acini. Bars represent 22.5 microns.