Cloned Stat5-bound chromatin fragments from PRL-stimulated T-47D breast cancer cells. Immunoprecipitated chromatin fragments were modified for cloning into a TA cloning vector and were transformed into electrocompetent bacteria. PCR was performed directly on white bacterial colonies using primers flanking the cloning site and was cycled 36 times. Samples were run on an agarose gel and analyzed for the presence of insert. (+) denotes vector control (no insert yields a vector-derived 172 bp product); (-) denotes negative PCR control.