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Figure 6 | Molecular Cancer

Figure 6

From: Stable low-level expression of p21WAF1/CIP1 in A549 human bronchogenic carcinoma cell line-derived clones down-regulates E2F1 mRNA and restores cell proliferation control

Figure 6

Proliferation kinetics for A549, and A549/p21 clone 1. Thirty mm Petri dishes were inoculated with 2 × 105 cells from either parental A549, clone 1, 29, 30, or 32, then incubated for 0, 24, 48, 72, or 96 hours. At each time point, cells were counted (total cell counts) and assessed for viability. Shown here are proliferation rates for parental A549 and A549/p21 clone 1 cell populations because while the growth rates are statistically significant between parental A549 and the A549/p21 clones, the proliferation rates among the clones were statistically insignificant. The experiment was repeated in triplicate and data reported as mean +/- standard deviation. The error bars are too small to visualize on this scale. The asterisk at 72 hrs indicates the time point when clonal populations were approximately 80% confluent and exhibited decreased viability.

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