Subcellular localization of m-aconitase. A-D. Immunofluorescent detection. LNCaP prostate cell line was probed with Mitotracker then fixed for immunofluorescence with Aconitase-2 Ab and visualized by Laser Scanning Confocal Microscope. A. no staining of either in nuclei (DAPI). B. red punctuate mitochondrial staining in cytoplasm (Mitotracker). C. green fluorescent of m-aconitase in cytoplasm (FITC). D. red and green fluorescence merge shows co-localization of m-aconitase in mitochondria. E. Immunocytochemical detection of m-aconitase. Note the dark brown punctuate mitochondrial staining in cytoplasm; and well defined clear nucleus.