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Table 1 Aconitase immunoreactivity of BPH glandular epithelumversus adenocarcinomatous glands of prostate cancer tissue sections.

From: Mitochondrial aconitase and citrate metabolism in malignant and nonmalignant human prostate tissues

 

TUMOR

ACONITASE IMMUNOREACTIVITY

ZIP1 IMMUNOREACTIVITY

CASE #

GRADE

BPH

PCA

BPH

Malignant

1

3

+++ 8.9

++ 6.4

++

Negative

2

3

+ 6.4

+++ 11.0

++

+

3

1

+ 6.2

+ 7.0

+

Negative

4

2

+++ 14.6

+++ 12.2

+

Negative

5

2

++ 12.6

+ 14.2

Negative

Negative

6

1

+++ 12.2

+++ 16.6

++

Negative

7

2

++ 10.0

+++ 15.6

+

+

8

1

+++ 11.4

++ 8.3

++

+

9

1

+ 7.5

+++ 18.0

++

Negative

10

2

+ 8.4

+ 8.6

++

Negative

11

1

+ 12.6

++ 11.8

++

Negative

12

2

+ 9.7

+++ 15.8

Negative

Negative

13

1

+++ 13.7

+++ 14.2

++

+

14

1

+++ 13.9

+++ 14.5

+

Negative

15

1

+ 7.6

+ 10.2

+

Negative

16

1

++ 11.6

+++ 13.9

+

Negative

17

1

++ 14.2

+++ 16.3

++

+

18

1

++ 9.2

++ 10.0

+

Negative

19

1

+++ 12.0

++ 10.9

+

Negative

20

2

+++ 14.7

+++ 18.2

++

+

21

1

+++ 14.1

+++ 17.6

Negative

Negative

22

3

+++ 16.3

+++ 17.9

-------------

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t-TEST- MEAN(SE)

 

POSITIVITY

2.1(0.85)

2.4(0.94)**

1.68(0.23)

0.32(0.10)*

QUANTITATION

11.3(2.9)

13. 1(3.8)**

-------------

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  1. Scoring of immunoreactivity: Negative, no positive cells;
  2. score +, <10% positive cells; score ++, 10–50% positive cells;
  3. score +++, > 50% positive cells.
  4. Quantitation = Average number of well defined ACON IHC dots per cell.
  5. Mean Positivity = sum of +'s/22
  6. ZIP1 data taken from Franklin et al [14].
  7. BPH vs Malignant *P < 0.01; **no significant difference