Figure 4

Expression of S3DN protein leads to apoptosis in SFM. SRB12-p9, Neo, S3DN2 and S3WT6 cells were cultured in 10% FCS-containing media or SFM for 4 days. (A) Cells and floating material were collected, fixed in 70% ethanol, stained with propidium iodide and analyzed by flow cytometry. The percentage of cells in the G1/0, S and G2 phases of the cell cycle are indicated for each cell line and treatment condition. Numbers to the left of the G1/0 peak indicate the percentage of sub-G1/0 particles from the total population of gate particles (excluding clumped cells). (B) Whole cell extracts were resolved on an SDS polyacrylamide gel, transferred to nitrocellulose and sequentially probed with antibodies specific for c-PARP, Bax and β-actin. Results shown in A and B are representative of 2 Neo and 3 S3DN and S3WT clones, in 2 independent experiments.