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Figure 1 | Molecular Cancer

Figure 1

From: Promoter hypermethylation-mediated inactivation of multiple Slit-Robo pathway genes in cervical cancer progression

Figure 1

Frequency of promoter hypermethylation of Slit-Robo pathway genes in cervical cancer progression. High molecular weight DNA isolated from pap smears and tissue sections was converted by sodium bisulphite [15]. MS-PCR was performed on converted DNA using primers specific to methylated and umethylated templates of each gene (Table 1). PCR products were separated on 2% agarose gels and visualized after ethidium bromide staining. Promoter methylation was scored on gels in the presence of positive and negative controls in each experiment. LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion. The total number of specimens analyzed in each type of tissue and gene are shown in parenthesis in the table below.

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Molecular Cancer

ISSN: 1476-4598