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Table 1 Primers used for MSP, RT-PCR, and cloning and sequencing.

From: Promoter hypermethylation-mediated inactivation of multiple Slit-Robo pathway genes in cervical cancer progression

MSP primers:

  

SLIT1-MF2

5'-TtcgTtcgcgagTTagacg-3'

19 bp

SLIT1-MR2

5'-aAAcgccgtcgcttAAaAA-3'

19 bp

SLIT1-UF2

5'-TgggTttgTgTgTggTgTTT-3'

20 bp

SLIT1-UR2

5'-ttttcctcctcAcaAcaAtcaA-3'

22 bp

SLIT2-MF

5'-gggaggcgggattgTTTag-3'

19 bp

SLIT2-MR

5'-catAAcgcgcgAAAAtAcac-3'

20 bp

SLIT2-UF

5'-gTgggaggTgggattgTTTa-3'

20 bp

SLIT2-UR

5'-AcctctccctcAccctcAac-3'

20 bp

SLIT3-MF

5'-ggtttcgtcgatggagttgt-3'

20 bp

SLIT3-MR

5'-aaacgcgtaaaacccgaaa-3'

19 bp

SLIT3-UF

5'-TGTGggTTagTGgggTTagg-3'

20 bp

SLIT3-UR

5'-cacaaacaaaacaaaacactcca-3'

23 bp

ROBO1-MF2

5'-cggcggcgatagTagTTaaa-3'

20 bp

ROBO1-MR2

5'-cgAAActAAAAAcgcccaAa-3'

20 bp

ROBO1-MF3

5'-cggcgtgcgTTTTTaTaatg-3'

20 bp

ROBO1-MR3

5'-gccAcgAAtAAcccgctAct-3'

20 bp

ROBO1-UF

5'-TggTggTaaagttggggtgt-3'

20 bp

ROBO1-UR

5'-ccAaAcccttcctccAAaAc-3'

20 bp

ROBO3-MF

5'-gcgggaTtTtTagTcggTTT-3'

20 bp

ROBO3-MR

5'-gAcctctccgcaAActAAcg-3'

20 bp

ROBO3-UF

5'-TggTgggaTtTtTagTTggTTT-3'

22 bp

ROBO3-UR

5'-ccAcaActtccccAcAAcAc-3'

20 bp

RT-PCR primers:

  

SLIT1-F

5'-ctggaactcaatggcaacaa-3'

20 bp

SLIT1-R

5'-acaaagcctggttgttctgg-3'

20 bp

SLIT2-F

5'-tcagctgtttcctgagttgc-3'

20 bp

SLIT2-R

5'-tggttgaaacttgccacaga-3'

20 bp

SLIT3-F

5'-gcgcctgaacaagaataagc-3'

20 bp

SLIT3-R

5'-ggatgcgactgatgttgttg-3'

20 bp

ROBO1-F1

5'-tgtttctggcccagcttatt-3'

20 bp

ROBO1-R1

5'-gtgttcaacaatgcgaggtg-3'

20 bp

ROBO1-F2

5'-aaatatggtgggcaaagctg-3'

20 bp

ROBO1-R2

5'-ctggatgactgtggtggttg-3'

20 bp

ROBO3-F

5'-gcagtcctccgtgatgattt-3'

20 bp

ROBO3-R

5'-ttggaggctacgcacacata-3'

20 bp

Cloning Primers:

  

SLIT2-cl-F

5'-gaattTaaagTTtgggaaaagttg-3'

24 bp

SLIT2-cl-R

5'-cttccaacaactactaaaatacaaaaa-3'

27 bp

SLIT2-cl-F3

5'-agtgTtgaTtagtggatatttTtgTT-3'

26 bp

SLIT2-cl-R3

5'-tcttctAtctcccaaAAatAaactt-3'

25 bp

  1. Nucleotides shown in upper case represent converted bases; MF and MR, methylation-specific forward and reverse primers; UF and UR, unmethylated forward and reverse primers; F and R, forward and reverse primers; cl-F and cl-R, cloning forward and reverse primers