DMC decreases the activity of the survivin promoter. Mass cultures of LN229 cells stably transfected with various luciferase reporter constructs under the control of either the survivin promoter (-6270Surv and -230Surv), the cyclin B promoter, or the cytomegalovirus (CMV) promoter, were treated with different concentrations of DMC for 36 hours. Thereafter, cellular lysates were analyzed for luciferase activity. For each reporter construct, basal level activity in the absence of drug at 36 hours was set to 100%. Shown is the mean (± SD; n = 3) luciferase activity from one experiment, which was repeated twice with similar results.