Figure 8

Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.