Table 2 Differential gene expression in parental HCT116 and 5-FU-resistant cell lines: additional affected regulatory pathways.
Gene symbol | par 8 hr 5 FUa | par 24 hr 5 FUa | ContinB 8 hr 5 FU a | ContinB 24 hr 5 FU a | ContinD 8 hr 5 FU a | ContinB as a result of 5 FU | altered in ContinD as a result of resistance development b | altered in ContinD as a result of resistance development b | Regulatory pathway/function |
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Nucleoside/nucleotide metabolism and nucleotide binding |
ADSS | -0.1 | -1.4 | 0.1 | -0.6 | -0.2 | -0.3 | 0.8 | 0.6 | GTP and nucleotide binding; nucleotide metabolism |
ATPIF1 | -0.2 | -1.0 | 0.4 | -0.1 | 0.0 | -1.5 | na | na | negative regulation of nucleoside metabolism |
ATAD2 | -0.7 | -0.5 | -0.6 | -0.6 | -0.3 | -1.2 | na | na | ATP and nucleotide binding; assembly/disassembly of protein complexes |
CMPK | 0.6 | 0.5 | 0.9 | 1.2 | 0.0 | 0.4 | 0.0 | 0.1 | nucleobase, nucleoside, nucleotide and nucleic acid metabolism |
GNL2 | -0.2 | 0.3 | -1.9 | -1.2 | -0.2 | -0.2 | 0.2 | 0.1 | nucleotide binding, protein binding, GTPase activity |
GNL3 | 0.1 | -0.2 | -0.5 | 0.0 | -0.4 | -1.7 | -0.5 | -0.3 | nucleotide binding, protein binding, GTPase activity |
MATR3 | -0.2 | -0.8 | -0.5 | -1.4 | -0.1 | 0.0 | -0.6 | -0.1 | RNA binding; metal ion binding; nucleotide and nucleic acid binding |
MBNL1 | -1.0 | -1.8 | 0.3 | -0.3 | -0.8 | -0.4 | na | na | nucleic acid binding; zinc ion binding |
PRPS2 | 0.1 | -0.3 | -1.6 | -1.2 | 0.0 | -0.2 | -0.2 | -0.2 | nucleoside metabolism; nucleotide biosynthesis |
TNRC8 | 0.4 | 0.7 | 0.6 | 1.3 | -0.2 | -0.1 | na | na | trinucleotide repeat containing 8 gene – function unknown |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | mRNA processing/transport |
EPRS | -0.3 | -1.5 | 0.2 | 0.0 | -0.7 | -0.1 | -0.9 | -0.6 | catalyzes aminoacylation of glutamic acid and proline tRNA species |
GNB2 | -0.5 | -2.2 | -0.3 | -0.6 | -0.1 | 0.3 | 0.1 | -0.2 | pre-mRNA processing and cytoskeleton assembly |
HMG20B | -0.6 | -2.1 | -0.7 | -0.4 | -0.2 | -0.2 | na | na | transcription factor; tRNA ligase activity; ATP binding |
SRRM1 | -0.6 | -1.7 | -0.4 | -0.2 | -0.3 | -0.4 | 0.8 | 0.1 | mRNA processing |
STAU1 | -0.3 | -1.2 | 0.3 | 0.0 | -0.1 | -0.4 | 0.4 | -0.1 | mRNA transport; intracellular transporter activity |
SYMPK | -1.9 | -0.9 | -0.4 | -0.8 | -0.3 | -0.3 | 0.3 | -0.1 | role in HSF1 modulation of Hsp70 mRNA polyadenylation |
THOC1 | -0.5 | -1.3 | -0.5 | -0.7 | -0.1 | -0.4 | -0.3 | 0.4 | regulates transcriptional elongation; mRNA export from nucleus |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Cytoskeleton/cell motility/cell adhesion |
ACTG2 | -0.1 | -1.3 | 0.0 | -0.6 | -0.1 | -0.4 | nd | nd | maintenance of the cytoskeleton; cell motility |
ARPC3 | 0.0 | -1.2 | -0.2 | -0.6 | 0.2 | -0.2 | 0.4 | 0.5 | regulation of actin cytoskeleton |
F2 | -0.4 | -1.2 | 0.4 | 0.1 | -0.1 | -0.6 | nd | nd | regulation of actin cytoskeleton; post-translational modification of proteins |
ILK | -0.4 | -0.3 | 0.0 | 0.7 | 0.1 | -1.1 | -0.2 | -0.3 | cell-matrix adhesion; integrin-mediated signaling pathway |
JUP | -0.4 | 0.2 | -1.1 | -0.5 | -0.1 | 1.1 | -0.8 | -0.3 | cell adhesion |
KRT7 | 0.1 | 0.4 | 0.8 | 1.4 | 0.3 | 0.4 | na | na | cytoskeleton organization and biogenesis; cell communication |
KRT8 | 0.3 | 0.1 | 1.0 | 1.7 | 0.3 | 0.6 | na | na | cytoskeleton organization and biogenesis; cell communication |
KRT19 | 0.3 | 0.7 | 1.0 | 1.7 | -0.1 | 0.5 | -0.3 | -0.9 | cytoskeleton organization and biogenesis; cell communication |
MAST1 | 0.2 | -0.7 | -1.4 | -1.3 | 0.1 | 0.2 | na | na | cytoskeleton organization and biogenesis; protein amino acid phosphorylation |
TUBB | 0.0 | -0.3 | 0.5 | 0.3 | -0.2 | -1.3 | na | na | microtubule-based movement; protein polymerization |
TUBE1 | -0.5 | -0.6 | 0.3 | -0.3 | -0.3 | -1.0 | na | na | microtubule-based movement; protein polymerization |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Amino acid, protein, carbohydrate metabolism |
AHCY | 0.7 | 0.9 | 1.3 | 1.4 | 0.2 | 0.3 | 0.2 | -0.3 | methionine, selenoamino acid metabolism |
GANAB | -0.2 | -0.5 | -1.4 | -1.3 | 0.1 | -0.2 | na | na | carbohydrate metabolism |
GCLC | -0.5 | -0.9 | -2.2 | -1.6 | -0.2 | -0.3 | na | na | glutamate metabolism |
GSS | -0.3 | -0.3 | -1.8 | -0.8 | 0.0 | 0.4 | -0.2 | -0.6 | glutamate and glutathione metabolism |
IVD | -0.7 | -0.2 | -1.6 | -2.4 | -0.3 | 0.3 | 1.0 | 0.1 | valine, leucine and isoleucine degradation |
MRP63 | 0.7 | 0.6 | 1.4 | 1.1 | 0.2 | 0.0 | na | na | protein synthesis within the mitochondrion |
OAT | 0.1 | -0.4 | -1.9 | -1.7 | -0.1 | 0.0 | -0.5 | 0.1 | arginine and proline metabolism; ornithine metabolism |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Signal transduction/transport |
CENTG3 | -1.1 | 0.2 | -0.5 | 0.2 | -0.6 | 1.1 | na | na | regulation of GTPase activity; small GTPase mediated signal transduction |
ERP70 | -0.2 | -1.9 | -0.9 | -0.4 | -0.1 | -0.3 | na | na | electron transport and protein secretion |
KPNA3 | -0.8 | -1.2 | -0.1 | -0.3 | -0.3 | -0.2 | -0.1 | -0.1 | involved in nuclear transport system; intracellular protein transport |
MTCH1 | 0.6 | 0.7 | 1.2 | 1.4 | 0.4 | 0.3 | 0.1 | 0.0 | neuronal ion channel clustering; transport; regulation of signal transduction |
NCBP1 | -0.3 | -0.6 | -1.1 | -1.0 | 0.0 | -0.3 | 0.0 | 0.0 | RNA splicing; mRNA nuclear export; mRNA processing; transport |
RAB8A | -0.3 | -2.4 | -0.1 | -0.7 | 0.1 | -0.2 | 0.5 | 0.1 | GTP/GDP-binding protein involved in protein transport |
RAN | -0.6 | -0.8 | -1.2 | -1.6 | 0.0 | -0.3 | na | na | RNA and protein export from nucleus; small GTPase mediated signal transduction; intracellular protein transport |
SLC16A1 | 0.2 | -1.3 | 0.5 | -0.5 | -0.1 | -0.7 | -0.1 | -0.5 | mevalonate and organic anion transporter activity |
SNAPAP | -0.4 | -0.9 | -0.9 | -1.6 | -0.1 | -0.2 | na | na | exocytosis; intracellular protein transport; neurotransmitter secretion |
TAX1BP1 | -0.6 | -0.1 | 0.6 | 0.6 | 0.2 | -1.1 | -1.3 | -0.7 | protein binding |
TAX1BP3 | -0.3 | 1.6 | -0.1 | 0.0 | 0.3 | -0.2 | na | na | ATP binding; signal transduction; ion transport |
TM9SF2 | -0.4 | -0.5 | -1.9 | -1.6 | -0.1 | -0.5 | 0.1 | 0.1 | transport |
TPD52 | -0.4 | -0.7 | -1.8 | -1.5 | -0.3 | -0.3 | na | na | calcium ion binding; protein binding; protein homodimerization activity; secretion |
UFM1 | -0.9 | -0.4 | -0.6 | -1.5 | 0.1 | 0.3 | na | na | ubiquitin cycle – function unknown |
VPS52 | -0.6 | -1.2 | 0.1 | -0.5 | 0.1 | -0.2 | 0.3 | -0.1 | involved in vesicle trafficking from endosomes to trans-Golgi network |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Oxygen metabolism |
COX5A | 0.8 | 0.4 | 1.2 | 1.0 | 0.2 | 0.1 | -0.1 | -0.1 | oxidative phosphorylation |
COX7C | 0.6 | 0.3 | 0.3 | 1.1 | 0.3 | 0.2 | na | na | oxidative phosphorylation |
MPV17 | -0.3 | 0.5 | -1.3 | 0.5 | -0.4 | 0.9 | -0.1 | -0.1 | oxygen and reactive oxygen species metabolism |
SDHB | -0.1 | -0.7 | -0.7 | -1.6 | 0.1 | -0.2 | -0.1 | -0.3 | oxidative phosphorylation; oxidative decarboxylation of pyruvate and TCA cycle |